Global gene expression analysis indicates that multiple pathways are affected during growth arrest of non-malignant human mammary epithelial cells in three dimensional cell cultures

The growth arrest of non-malignant human mammary epithelial cells (HMEC) in Matrigel provides a three dimensional model system in which to study a normal cell process that is disrupted in cancer. To systematically probe the molecular changes that lead to growth arrest in this system, we have analyzed the expression profiles of 22,283 gene transcripts using Affymetrix HG-U133A microarrays. By these criteria, 354 genes, including many involved in cell cycle transit, cytokinesis, and mitosis, were found to be down-regulated, and 105 genes, including many involved in epidermal differentiation, inflammatory processes, and negative regulation of cell proliferation, were found to be up-regulated.

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2018-04-20T21:06:48+00:00