Isolation and detection of breast cancer cells in blood samples using antibody cocktails selected by large-scale genome-wide screening

Screening and prevention is critical to reducing breast cancer deaths, and mammography is not an ideal tool. To address this critical unmet need, we developed a panel of cell surface markers whose expression patterns specifically distinguish breast cancer cells in blood samples. These genes were selected based on a discovery platform tailored to detect early-stage breast cancers and include 20 novel putative cell surface molecules that are candidates for breast cancer cell detection and isolation. The innovation of this strategy is twofold. First, it uses a panel of molecules instead of an individual molecule to detect and isolate breast cancer cells and, second, it relies on molecules that are clinically relevant to breast cancers, rather than relying on generic epithelial cell markers.

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